0036 ARC Genotype Modulates REM EEG Spectral Power Following Total Sleep Deprivation

Abstract Introduction EEG spectral power in the alpha range is indicative of a wake-like state, with typically high during wakefulness and reduced sleep. We previously reported that single nucleotide polymorphism (SNP) activity-regulated cytoskeleton associated protein (ARC) gene modulates non-REM theta power. Here we sought to determine whether ARC genotype also phenotypic differences REM Methods 43 healthy adults (27.6±4.8y; 23 females) participated one two in-laboratory studies. Each participant had 10h baseline sleep opportunity (22:00–08:00), 38h TSD, recovery (22:00–08:00). Sleep periods were recorded polysomnographically visually scored according AASM criteria. Genomic DNA was assayed for c.*742 + 58C>T non-coding SNP, rs35900184. Log-transformed (C3-M3 derivation) over 0.2 Hz frequency bins each four bands – delta (0.8–4.0 Hz), (4.2–8.0 (8.2–12.0 beta (12.2–16.0 Hz) analyzed by band using mixed-effects ANOVA fixed effects genotype, night (baseline, recovery), bin, their interactions. Analyses included study age as covariates random effect subjects on intercept. Results The distribution this sample 28 C/C homozygotes, 10 C/T heterozygotes, 5 T/T homozygotes. There significant interaction (F2,1560=23.80, p< 0.001). Compared sleep, homozygotes 13.0% more post-TSD whereas 1.2% less heterozygotes 3.2% This differential TSD genotypes not seen other bands. Conclusion Our results show mediates response displaying pronounced increase following deprivation. SNP has been schizophrenia, psychiatric disorder known disturbances. As such, ARC-mediated might be marker psychosis-like predisposition. Support (if any) ONR N00014-13-1-0302; NIH R21CA167691; USAMRDC W81XWH-18-1-0100; ARO W911NF2210223